Histological examination of gastric biopsy material stained for Helicobacter pylori (according to Romanovsky–Gimze)
Histological examination of gastric biopsy material stained for Helicobacter pylori (according to Romanovsky–Giemsa) is a method for detecting Helicobacter pylori, which allows not only to identify the bacterium, but also to assess the condition of the mucous membrane, the degree of inflammation, and the presence of precancerous changes.
The procedure involves taking a tissue sample during endoscopy and then preparing thin sections for microscopy.
Romanowsky-Giemsa staining, the mixture effectively detects microorganisms and cellular elements, which increases the accuracy of the diagnosis. Histological examination is considered one of the most accurate methods for detecting H. pylori. It allows not only the detection of bacteria, but also the assessment of the degree of inflammation (gastritis), the presence of intestinal metaplasia, and other pathological changes that can lead to stomach cancer.
Clinical indications:
Suspected inflammatory diseases of the stomach and duodenum:
- chronic gastritis, including superficial, atrophic, and erosive;
- gastric and duodenal ulcers;
- reflux gastritis.
Presence of complaints and clinical signs indicating possible H. pylori infection:
- pain and discomfort in the epigastric region;
- heartburn, belching, nausea;
- indigestion of unknown origin.
To clarify morphological changes in the gastric mucosa:
- endoscopically detected erosions, ulcers, hyperemia, mucosal edema;
- suspected precancerous conditions: intestinal metaplasia, dysplasia, mucosal atrophy.
Monitoring after treatment:
- to control the effectiveness of H. pylori eradication therapy (6-12 months after treatment).
As part of a comprehensive examination of patients with a heightened risk of cancer:
- since persistent H. pylori infection is a risk factor for the development of malignant tumors.