Determination of autoimmune Ig G to double-stranded DNA in blood serum by indirect immunochemiluminescence

Autoimmune IgG to double-stranded DNA is intended for in vitro metering of double-stranded DNA IgG antibodies (dsDNA) in human serum and plasma, as an aid in the clinical diagnosis of systemic lupus erythematosus (SLE).

IgG autoantibodies are components of the immune system whose function fails for an unspecified reason, resulting in immune assault against the body. In this case, the assault is expressed through the production of IgG immunoglobulin to double-stranded DNA, a specific protein that, in contact with strictly defined elements, destroys them.

Thus, the test has found wide application in rheumatology, the branch of medicine that studies autoimmune diseases. Antinuclear antibodies (ANA) detection is of central importance for the clinical diagnosis of connective tissue diseases.

For the diagnosis of systemic lupus erythematosus (SLE), dsDNA antibodies are considered a highly specific marker representing one of the diagnostic criteria for SLE. More than 90% of sera from patients with active SLE contain dsDNA antibodies. In addition, dsDNA antibodies detection is a tool for monitoring the clinical course of SLE, as there is a clear correlation between anti-dsDNA capacity and disease activity, in particular renal damage.