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Determination of benzodiazepines and their derivatives in urine (chromatography)

Benzodiazepines are psychoactive drugs widely used to treat anxiety, insomnia, and a number of other conditions. Due to their sedative effect, abuse is possible, making urine testing for these substances particularly important for detecting such cases.

The test is performed using high-performance liquid chromatography with a mass spectrometric detector (HPLC-MS). This method is highly sensitive and allows for the reliable detection of both the parent substances and their biochemical metabolites.

The analysis is aimed at detecting a number of benzodiazepines and their metabolites, including:

  • Diazepam
  • Alprazolam
  • Clonazepam
  • Lorazepam
  • Oxazepam
  • Nitrazepam