Determination of nicotine and its metabolites
Analysis for nicotine and its metabolites (e.g., cotinine) allows the detection of tobacco product or e-cigarette use. This test is used to assess the impact of nicotine on the body and to control compliance with anti-smoking laws.
Method: HPLC-MS/MS with ion trap (qualitative detection)
For qualitative analysis, HPLC-MS/MS with ion trap is used to confirm or rule out the presence of nicotine and its metabolites in urine.
Analyzed substances: nicotine, cotinine (main metabolite), anabazine